Extended Data Fig. 2: Medullary localization of DCLK1bright cells and KRT10 bodies.
From: Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development
Representative confocal maximum projections (25 μm) of immunofluorescence staining of thymic slices at low magnification. a, KRT5 (red) and KRT10 (green) (left) and KRT5 (red) and DCLK1 (green) (right) showing medullary localization of KRT10 and DCLK1. b, Left, KRT5 (red) and DCKL1 (green). Right, DCLK1 alone. Region was selected for presence of multicellular DCLK1bright cell clumps as indicated by white arrows. Scale bars, 100 μm. n = 3 mice; images are representative of three independent experiments.
