Extended Data Fig. 7: Comparing intestinal tuft cells and the mTEC IV population.
From: Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells
a, Flow cytometry scheme of mTEC IV cells (Sox9-eGFP+L1CAM+) sorting. b, Flow cytometry scheme of small intestine Hpgds-tdTomato+ tuft cell sorting. c, Heat map showing gene expression profiles across 1,903 intestinal tuft (Hpgds-tdTomato+) single cells, grouped into 68 metacells. d, Comparison of gene expression between tuft cells isolated from small intestine (Hpgds-tdTomato+; x axis) and mTEC IV cells isolated from thymus (CD45−EpCAM+Sox9-eGFP+L1CAM+; y axis). Axes represent UMI count per 1,000 UMI, normalized to cell numbers. e, Normalized mean expression of differential genes across TEC populations, sorted mTEC IV cells (L1CAM+ Sox9-eGFP+) and intestinal Tuft (Hpgds-tdTomato+) cells. f, GO annotations enrichment in differential genes (fold change >2) between intestinal tufts (n = 634) and mTEC IV cells (L1CAM+Sox9-eGFP+) (n = 1,308).
