Extended Data Fig. 8: The transcription factor Pou2f3 is a master regulator of mTEC IV.
From: Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells
a, Flow cytometry scheme for sorting of EpCAM+ cells from Pou2f3 knockout thymi. b, Projection of representative TEC subtype-specific markers onto the two-dimensional mapping of Pou2f3 wild-type and knockout cells to the epithelial model of Fig. 1 (see Methods). c, Bar plot showing log2 fold change between TEC subpopulation abundances in Pou2f3 knockout (n = 451 single cells) and wild-type (n = 1121) mice. Error bars represent 95% confidence intervals. d, Pooled expression of mTEC IV genes across cells from Pou2f3 knockout and wild-type (WT) mice. The x axis represents UMI count per 1,000 UMI; y axis represents fraction of expression from total UMI count of the cells. Green cells indicate cells classified as mTEC IV (two-sided binomial test; FDR-adjusted P < 10−10). n = 1,572 single cells. e, Differential gene expression between mTEC I–III cells isolated from control (wild-type) and Pou2f3 knockout mice. Axes represent UMI count per 1,000 UMI, normalized to cell numbers.
