Extended Data Fig. 1: Single-cell data quality controls.
From: Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells
a, Summary of all single cells analysed in this study, divided into experimental procedures. ‘nbatches’ indicates number of technical replicates; ‘ncells’ indicates number of cells after filtering (see Methods). b–e, Colour-coded tracks summarizing the number of Illumina reads per cell (b), transcripts (UMI) detected in each cell (c), fraction of analysed cells from each amplification batch (d) and estimation of technical noise for each amplification batch (e). Cells are coloured by experimental procedure. Technical noise is assessed by genomic UMIs in empty wells as previously described4 (see Methods).
