Extended Data Fig. 6: Upregulation of ISGs in HeLa and murine cells following loss of PNPase accentuated by mitochondrial outer membrane permeabilization.
From: Mitochondrial double-stranded RNA triggers antiviral signalling in humans
a, Heat map of ISGs generated from a subset extracted from a list of significantly expressed genes in siRNA-treated HeLa cells. Gene expression is depicted by colour intensity. Green denotes upregulation and red downregulation. b, RT–qPCR analysis of IFNB1 mRNA in HeLa cells treated with indicated siRNAs and then 8 h of treatment with vehicle or ABT-737. Data are the mean of two independent experiments. c, Western-blot analysis of the cytochrome c (cyt c) release into the cytoplasm of HeLa cells treated with vehicle or ABT-737 for 8 h. Subcellular fractionation purity confirmed by relevant markers. Blots are representative of two experiments. d, log2(fold change) expression levels of ISGs and genes involved in interferon signalling in HepKO versus wild-type female mice. ISG list is based on the Reactome database33. e, Western blot of whole-cell extracts from cells treated with the indicated siRNAs. Blots are representative of two experiments. For gel source data, see Supplementary Fig. 1.
