Extended Data Fig. 3: Unwinding activity of SUV3 is required to suppress mtdsRNA accumulation.
From: Mitochondrial double-stranded RNA triggers antiviral signalling in humans
a, Left, confocal images of HEK 293 cells expressing stably integrated wild-type SUV3 (hSUV3_WT) and the catalytically inactive (G207V) dominant negative form (hSUV3_G207V) stained with J2 ab (green). Mitochondria stained with MitoTracker Deep Red (red). Nuclei stained with Hoechst (blue). Right, quantitative analysis of fluorescence signal from HEK 293 cells in the above experiment. Data are mean ± s.e.m. from 16 cells. b, Top, northern blots of J2 immunoprecipitated dsRNA from hSUV3_WT and hSUV3_G207V overexpressing HEK 293 cell lines with four different probes spanning the entire mitochondrial genome. Bottom, diagram depicts positions of probes on mitochondrial genome. Blots are representative of two experiments. For gel source data, see Supplementary Fig. 1.
