Fig. 1: Frequent large deletions detected in mouse zygote injections. | Nature

Fig. 1: Frequent large deletions detected in mouse zygote injections.

From: Large deletions induced by Cas9 cleavage

Fig. 1

a, The number of founders that contain large deletions detected by large PCR (~1.6 kb). Asterisk symbol indicates ~3.2-kb PCR was also performed. b, Sequencing of large deletion bands after ~1.6-kb PCR from mouse zygote injection samples. ‘0’ represents the cutting site of Cas9. Each bar represents the deletion position relative to the corresponding NGG PAM sequence. c, Whole-genome sequencing analysis of Rsad2-gRNA-treated mouse ES cell pools. Integrative genome viewer snapshot shows reads paired in sequencing and sorted by insert size. Read pairs in red indicate discordantly mapping pairs indicative of large deletions.

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