Extended Data Fig. 6: Growth competition between SY14/SY14^a and BY4742/BY4742^a diploid cells.

a, Blue circles represent BY4742/BY4742^a (with HIS3 marker) cells that could grow only on SC-His plates; pink triangles represent SY14/SY14^a (with URA3 marker) cells that could grow only on SC-Ura plates; green diamonds represent ‘fusion’ cells of BY4742/BY4742^a and SY14/SY14^a that could grow on both SC-His and SC-Ura plates. Data from three biological replicates are presented. b, FACS analysis of DNA content of BY4742/BY4742^a and SY14/SY14^a diploid cells before and after co-culture. Data are representative of two independent experiments. c, PCR verification of genomes from BY4742/BY4742^a and SY14/SY14^a diploid cells. H1–H3: colonies grown only on SC-His plates; HU1–3: colonies grown on both SC-His and SC-Ura plates. The BY4742/BY4742^a and SY14/SY14^a diploid cells before co-cultivation were used as control. Two pairs of primers, specific for genomes of BY4742/BY4742^a and SY14/SY14^a, were used. Data shown are representative images of two independent experiments.