Extended Data Fig. 4: WM-8014 causes cell cycle exit and senescence in MEFs, but not DNA damage or cell death.
From: Inhibitors of histone acetyltransferases KAT6A/B induce senescence and arrest tumour growth
a, Assessment of DNA damage using flow cytometry to detect γH2A.X. Top, exposure of MEFs to ultraviolet light (positive control). Bottom, experimental samples. Quantification is displayed in the bar graph. b, Flow-cytometry gating strategy for cell death analysis and representative experimental samples. Negative and positive controls (untreated and ultraviolet-light-irradiated cells, respectively) are shown in the left panels. Annexin V marks phosphatidylserine externalization on cells undergoing apoptosis, propidium iodine (PI) uptake marks cells undergoing other forms of cell death, annexin V/PI double-positive staining marks cells in late-stage apoptosis. n = 3 cultures (a, b). Data are mean ± s.e.m. and were analysed by one-way ANOVA with treatment as the independent factor.
