Extended Data Fig. 7: The formation of glioma as observed in serial sections of mouse brain.
From: Human glioblastoma arises from subventricular zone cells with low-level driver mutations
a, Images from the P53/PTEN/EGFR-mutant mice show that the tdTomato-positive cells initially locate in the rostral SVZ, where mutations are edited. Over time, these cells migrate to distant cortical regions and proliferate to form the tumour. b, Images from wild-type mice at 16 weeks after the electroporation of the plasmid containing sgRNA for lacZ gene with the expression of Cas9/Cre as a control. In panels a and b, section points were the middle of the olfactory bulb at 0.7, −1, −2.5 and −3.5 mm apart from bregma; time intervals were 2 days, 8 weeks, 13 weeks and 16 weeks after electroporation. Scale bars, 500 μm. c, Quantification of tdTomato signal intensities corresponding to the average of cortical regions from four serial sections in the affected side. Section points were 0.7, −1, −2.5 and −3.5 mm apart from bregma. Control indicates the wild-type mouse electroporated with the plasmid containing sgRNA for the lacZ gene with the expression of Cas9/Cre. *P = 0.007, **P = 0.0002 (n = 6 for control and mutant mice at each time point), Student’s two-tailed t-test. Error bars represent mean ± s.e.m.
