Extended Data Fig. 9: Aberrant growth of OPC lineage in the distant region.
From: Human glioblastoma arises from subventricular zone cells with low-level driver mutations
a, The scatter dot graph shows the percentage of cells positive for various NSC-derived cell lineage markers, such as NeuN for neurons, MBP for oligodendrocytes, GFAP for astrocytes, and PDGFRα and OLIG2 for oligodendrocyte-progenitor cells (OPCs). The average of four representative cortical regions at the caudal cortex (−3.5 mm apart from bregma) away from the mutation-arising SVZ were analysed (n = 6 for control and mutant mice). Error bars represent mean ± s.e.m. b, Representative immunostaining images of OLIG2-, PDGFRα-, GFAP- and tdTomato-positive cell regions at the caudal cortex (−3.5 mm apart from bregma). White arrows indicate tdTomato-positive cells co-stained with OLIG2 or PDGFRα. Scale bars, 50 µm. n = 6 mice c, Immunostaining of Ki67, a marker of proliferation, and cell-type markers PDGFRα, OLIG2 and GFAP in P53/PTEN/EGFR-mutant mice before the formation of a visible glioma. White arrows indicate Ki67-positive cells. Scale bars, 50 μm. n = 3 mice. d, Illustration of the progress of migration and tumour development via the aberrant growth of OPCs.
