Extended Data Fig. 4: Schematic of the experimental procedures for single-cell sequencing of tumour-private mutations and shared driver mutations in frozen tissue. | Nature

Extended Data Fig. 4: Schematic of the experimental procedures for single-cell sequencing of tumour-private mutations and shared driver mutations in frozen tissue.

From: Human glioblastoma arises from subventricular zone cells with low-level driver mutations

Extended Data Fig. 4

Nuclei are isolated from homogenized tissue and stained with DAPI. Flow cytometry sorts DAPI-stained nuclei as a single nucleus into each well of a 96-well plate, which is confirmed by fluorescent microscopy. Then, single-cell PCR followed by Sanger sequencing is performed.

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