Extended Data Fig. 5: Mei3–Mei2, but not sme2–Mei4, signalling suppresses mating in zygotes and prevents polyploid formation. | Nature

Extended Data Fig. 5: Mei3–Mei2, but not sme2–Mei4, signalling suppresses mating in zygotes and prevents polyploid formation.

From: Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization

Extended Data Fig. 5

a, Time-lapse showing re-fusion of mei3Δ homothallic cells. b, Incidence of re-fertilization and zygotic growth in mating mixtures of homothallic strains with indicated genotypes. c–g, Time-lapse showing re-fusion of PiΔ, MiΔ, mei2Δ, mei2F644A and mei2Δmei3Δ homothallic cells, as indicated. In e, fusion is evidenced through transfer of M-cell-expressed GFP. In g, fusion is evidenced through transfer of M-cell-expressed sfGFP and P-cell-expressed mCherry. h, i, Time-lapse showing diploid zygote formation in sme2Δ and mei4Δ homothallic cells, as indicated. Note that zygotes do not exhibit any growth or attempt mating with other cells.

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