Extended Data Fig. 5: BiFC visualization of SLR1–GIF1–GRF4 interactions.
From: Modulating plant growth–metabolism coordination for sustainable agriculture
a, Details of constructs expressing GRF4 and variants deleted for specific domains. GRF4 contains the QLQ and WRC domains, positions as indicated. b, BiFC assays. Constructs expressing GRF4 or deletion variants (shown as in a) tagged with the N terminus of YFP were co-transformed into tobacco leaf epidermal cells, together with constructs expressing GIF1 or SLR1 tagged with the C terminus of YFP, respectively. Scale bars, 60 μm. c, BiFC assays. Constructs expressing GRF1 or related GRFs and GIFs family proteins tagged with the N terminus of YFP were co-transformed into tobacco leaf epidermal cells together with a construct expressing SLR1 tagged with the C terminus of YFP. Scale bar, 60 μm. b, c, Images of BiFC assays are representative of three experiments performed independently with similar results.
