Extended Data Fig. 6: SLR1 inhibits GRF4–GIF1 self-promotion of GRF4 mRNA and GRF4 protein abundance.
From: Modulating plant growth–metabolism coordination for sustainable agriculture
a, GFR4 mRNA abundance, plant genotypes as indicated. Abundances shown are relative to NJ6 (set to 1). b, The effects of GA and PAC on GRF4 mRNA abundance in two-week-old NJ6 plants. Abundances shown are relative to the water treatment control (set to 1). c, ChIP–PCR GRF4-mediated enrichment (relative to input) of GCGG-containing GRF4 promoter fragments (marked with asterisks). d, GRF4-activated promotion of transcription from the GRF4 gene promoter-luciferase reporter construct is enhanced by GIF1 and inhibited by SLR1. Luciferase/renilla activity shown relative to the empty vector control (set to 1). a–d, Data are mean ± s.e.m. (n = 3). Different letters denote significant differences (P < 0.05) from Duncan’s multiple range tests. e, GRF4 abundance (as detected by an anti-GRF4 antibody), plant genotypes as indicated. HSP90 serves as loading control. Blots are representative of three experiments performed independently with similar results.
