Extended Data Fig. 2: Comparisons NJ6, NJ6-sd1 and NJ6-sd1-GRF4ngr2 isogenic line traits reveals that GRF4 regulates expression of \({{\bf{NH}}}_{{\bf{4}}}^{{\boldsymbol{+}}}\)-metabolism genes.
From: Modulating plant growth–metabolism coordination for sustainable agriculture
a, Mature plant height. Data are mean ± s.e.m. (n = 16). b, The number of tillers per plant. Data are mean ± s.e.m. (n = 16). c, The number of grains per panicle. Data are mean ± s.e.m. (n = 16). d, Flag-leaf width. Data are mean ± s.e.m. (n = 16). e, Culm (stem) width expressed as diameter of the uppermost internode. Data are mean ± s.e.m. (n = 16). f, Grain yield per plant. Data are mean ± s.e.m. (n = 220). g, Relative root abundance of AMT1.2 mRNA in NILs, genotypes as indicated. Abundances shown are relative to NJ6 plants (set to 1). Data are mean ± s.e.m. (n = 3). h, Root glutamine synthase (GS) activities. Data are mean ± s.e.m. (n = 3). i, Relative shoot abundance of Fd-GOGAT mRNA. Abundances shown are relative to NJ6 plants (set to 1). Data are mean ± s.e.m. (n = 3). j, Shoot glutamine synthase (GS) activities. Data are mean ± s.e.m. (n = 3). k–n, Flag–GRF4-mediated ChIP–PCR enrichment (relative to input) of GCGG-containing promoter fragments (marked with an asterisk) from AMT1.2, GS2, NADH-GOGAT2 and Fd-GOGAT promoters. Diagrams depict putative AMT1.2, GS2, NADH-GOGAT2 and Fd-GOGAT promoters and fragments (1–6). Data are mean ± s.e.m. (n = 3; panels k–n). a–n, Different letters denote significant differences (P < 0.05) from a Duncan’s multiple range test. o, GRF4 activates AMT1.2, GS2, NADH-GOGAT2 and Fd-GOGAT promoter–luciferase fusion constructs in transient transactivation assays. Data are mean ± s.e.m. (n = 3). **P < 0.05 compared to control group by two-sided Student’s t-tests.
