Extended Data Fig. 3: GRF4 regulates expression of multiple \({{\bf{NO}}}_{{\bf{3}}}^{{\boldsymbol{-}}}\) metabolism genes.
From: Modulating plant growth–metabolism coordination for sustainable agriculture
a, Relative abundance of NRT1.1B, NRT2.3a and NPF2.4 mRNAs that encode \({{\rm{NO}}}_{3}^{-}\) uptake transporters. Abundances shown are relative to NJ6 (set to 1). Data are mean ± s.e.m. (n = 3). b, Relative abundances of NIA1, NIA3 and NiR1 mRNAs that encode \({{\rm{NO}}}_{3}^{-}\)-assimilation enzymes. Abundances shown are relative to NJ6 (set to 1). Data are mean ± s.e.m. (n = 3). c–h, Flag–GRF4-mediated ChIP–PCR enrichment (relative to input) of GCGG-containing fragments (marked with asterisks) from promoters of NRT1.1B (c), NRT2.3a (d) and NPF2.4 (e) genes that encode \({{\rm{NO}}}_{3}^{-}\)-uptake transporters and NIA1 (f), NIA3 (g) and NiR1 (h) genes that encode \({{\rm{NO}}}_{3}^{-}\)-assimilation enzymes. Data are mean ± s.e.m. (n = 3). a–h, Different letters denote significant differences (P < 0.05) from a Duncan’s multiple range test. i, GRF4 activates NRT1.1B, NRT2.3a, NPF2.4, NIA1, NIA3 and NiR1 promoter–luciferase fusion constructs in transient transactivation assays. Data are mean ± s.e.m. (n = 3) in all panels. P values are from a two-sided Student’s t-test.
