Extended Data Fig. 5: Optogenetic activation of DR 5-HT neurons or DR-to-NAc 5-HT terminals rescues social deficits induced by 16p11.2 deletion, but does not alter control behaviours.
From: 5-HT release in nucleus accumbens rescues social deficits in mouse autism model
a, b, Quantification of chamber and proximity time in the three-chamber assay (a: eYFP, F2,21 = 84.95, P < 0.001, n = 8 (left); F1,14 = 44.00, P < 0.001, n = 8 (right); b: ChR2, F6,81 = 9.26, P < 0.001, n = 10 (left); F3,54 = 11.54, P < 0.001, n = 10 (right)). c–e, Quantification of the novel object interaction assay (c: F1,32 = 0.03819, P = 0.8463, n = 9), locomotion assay (d: F1,32 = 0.141, P = 0.7097, n = 8–10), and centre time (e: F5,80 = 0.195, P = 0.9636, n = 8–10) in Sert-cre:16p11.2flx mice expressing DIO-eYFP or DIO-ChR2 in DR receiving soma stimulation. f, g, Quantification of chamber and proximity time in the three-chamber assay (f: eYFP, F2,27 = 73.89, P < 0.001, n = 10 (left); F1,18 = 63.38, P < 0.001, n = 10 (right); g: ChR2, F6,81 = 11.33, P < 0.001, n = 10 (left); F3,54 = 14.55, P < 0.001, n = 10 (right)). h–j, Quantification of novel object interaction assay (h: F1,36 = 0.01038, P = 0.9194, n = 10), locomotion assay (i: F1,32 = 0.3655, P = 0.5497, n = 9), and centre time (j: F5,90 = 0.9092, P = 0.4788, n = 10) in Sert-cre:16p11.2flx mice expressing DIO-eYFP or DIO-ChR2 in DR receiving DR-to-NAc 5-HT terminal stimulation. Data are mean ± s.e.m. ***P < 0.001; repeated measures, two-way ANOVA with Tukey’s multiple comparison post hoc test. Comparisons with no asterisk had P > 0.05 and were considered not significant.
