Extended Data Fig. 6: 5-HT release in the NAc is not acutely reinforcing.
From: 5-HT release in nucleus accumbens rescues social deficits in mouse autism model
a, b, Quantification of chamber time in the real-time CPP assay for 0 Hz (a) and 20 Hz (b) stimulation for initial (left) and reversal (right) stimulations (a: 0 Hz initial F2,16 = 10.22, P < 0.05, n = 9; 0 Hz reversal F2,16 = 29.01, P < 0.001, n = 9; b: 20 Hz initial F2,16 = 19.37, P < 0.001, n = 9; 20 Hz reversal F2,16 = 7.53, P < 0.01, n = 9) in Sert-cre mice receiving DR-to-NAc terminal stimulation. c, d, Quantification of chamber time in the real-time CPP assay for 0 Hz (c) and 20 Hz (d) stimulation for initial (left) and reversal (right) stimulations (c: 0 Hz initial F2,16 = 12.44, P < 0.001, n = 9; 0 Hz reversal F2,16 = 19.92, P < 0.001, n = 9; d: 20 Hz initial F2,16 = 8.56, P < 0.01, n = 9; 20 Hz reversal F2,16 = 9.517, P < 0.001, n = 9) in Sert-cre:16p11.2flx mice receiving DR-to-NAc terminal stimulation. e, Quantification of nosepokes for active and inactive ports for Sert-cre and Sert-cre:16p11.2flx mice (Sert-cre F2,32 = 1.9, P = 0.1655, n = 9; Sert-cre:16p11.2flx F2,32 = 0.25, P = 0.7821, n = 9). f, g, Quantification of chamber time (left) and proximity time (right) in high-fat food three-chamber assay for Sert-cre mice (F6,72 = 0.6713, P = 0.6731, n = 9; F3,48 = 1.495, P = 0.2279, n = 10) and Sert-cre:16p11.2flx mice (F6,72 = 0.4006, P = 0.8763, n = 10; F3,48 = 0.4157, P = 0.7425, n = 10) (g). Post-hoc analysis showed no significant difference between stimulated and non-stimulated chambers. *P < 0.05, **P < 0.01, ***P < 0.001; repeated measures, one-way (a–d) or two-way (f, g) ANOVA with Tukey’s multiple comparison post hoc test, or one-way ANOVA with Sidak’s multiple comparison post hoc test comparing active to inactive nosepokes (e). Comparisons with no asterisk had P > 0.05 and were considered not significant.
