Extended Data Fig. 7: Activation of DR 5-HT terminals in the dorsal striatum does not enhance sociability nor rescue social deficits induced by 16p11.2 deletion.
From: 5-HT release in nucleus accumbens rescues social deficits in mouse autism model
a, Schematic of DIO-ChR2-eYFP injected into the DR and optic fibre implanted above the dorsal striatum (DS) in Sert-cre or Sert-cre:16p11.2flx mice. b, Timeline of behavioural experiments. c, d, Quantification of sociability during the juvenile interaction assay (c: F1,14 = 0.5429, P = 0.4734, n = 4–5) and sociability in the three-chamber assay (d: F1,14 = 0.055, P = 0.8177, n = 4–5) in Sert-cre or Sert-cre:16p11.2flx mice expressing DIO-eYFP or DIO-ChR2 with terminal stimulation of DR 5-HT neurons in the dorsal striatum. e, f, Quantification of the three-chamber assay showing chamber (left) and proximity (right) time (e: Sert-cre, F6,36 = 1.078, P = 0.3939, n = 5 (left); F3,24 = 2.009, P = 0.1395, n = 5 (right); f: Sert-cre:16p11.2flx, F2,9 = 16.44, P < 0.001, n = 4 (left); F1,6 = 11.2, P < 0.01, n = 4 (right)). g–i, Quantification of novel object interaction assay (g: F1,14 = 0.058, P = 0.8125, n = 4–5), locomotion assay (h: F1,14 = 0.6195, P = 0.4444, n = 4–5), and centre time (i: F1,7 = 8.292, P < 0.05, n = 4–5) in Sert-cre or Sert-cre:16p11.2flx mice. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001; repeated measures, two-way ANOVA with Tukey’s multiple comparison post hoc test. Comparisons with no asterisk had P > 0.05 and considered were not significant. The schematic of the mouse brain in this figure has been adapted with permission from Franklin & Paxinos46.
