Extended Data Fig. 1: Activation of DR neurons or their NAc projections increases sociability, and bidirectional modulation of DR 5-HT neuron activity bidirectionally modifies sociability.
From: 5-HT release in nucleus accumbens rescues social deficits in mouse autism model
a, b, Quantification of chamber time (a: eYFP, F6,45 = 0.6823, P = 0.6647, n = 6; ChR2, F6,72 = 17.21, P < 0.01; n = 9) and proximity time (b: eYFP, F3,30 = 0.7517, P = 0.5300, n = 6; ChR2, F3,48 = 32.96, P < 0.01, n = 9) in the three-chamber assay. c, Quantification of centre time in the locomotion assay (c: F5,65 = 1.263, P = 0.2908, n = 6–9). d, e, Quantification of chamber time (d: eYFP, F6,54 = 0.2602, P = 0.9529, n = 7; ChR2, F6,72 = 19.36, P < 0.01, n = 9) and proximity time (e: eYFP, F3,36 = 0.3456, P = 0.7925, n = 7; ChR2, F3,48 = 26.44, P < 0.01, n = 9) in the three-chamber assay. f, Quantification of centre time in the locomotion assay (F5,40 = 0.7786, P = 0.5710, n = 5). g, h, Quantification of chamber time (g: eYFP, F6,54 = 0.7293, P = 0.6280, n = 7; ChR2, F6,72 = 3.812, P < 0.05, n = 9) and proximity time (h: eYFP, F3,36 = 0.9256, P = 0.4383, n = 7; ChR2, F3,48 = 4.844, P < 0.05, n = 9) in the three-chamber assay. i, j, Quantification of chamber time (i: eYFP, F6,54 = 1.058, P = 0.3989, n = 7; NpHR, F6,81 = 13.04, P < 0.01, n = 10) and proximity time (j: eYFP, F3,36 = 1.661, P = 0.1926, n = 7; NpHR, F3,54 = 16.29, P < 0.01, n = 10) in the three-chamber assay. k, l, Quantification of novel object interaction assay (k: F1,52 = 0.01018, P = 0.9200, n = 13–15), locomotion assay (l: F1,52 = 0.7626, P = 0.3865, n = 13–15), and centre time (m: F5,130 = 0.766, P = 0.5759, n = 13–15) in Sert-cre mice expressing DIO-eYFP or DIO-ChR2 in DR receiving soma stimulation. n–p, Quantification of novel object interaction assay (n: F1,30 = 0.04112, P = 0.8407, n = 7–10), locomotion assay (o: F1,30 = 0.3837, P = 0.5403, n = 7–10), and centre time (p: F5,80 = 1.195, P = 0.3190, n = 8–10) in Sert-cre mice expressing DIO-eYFP or DIO-NpHR in DR receiving soma stimulation. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, two-way ANOVA with Tukey’s multiple comparison post hoc test. Comparisons with no asterisk had P > 0.05 and were considered not significant.
