Extended Data Fig. 2: Bidirectional modulation of DR-to-NAc 5-HT terminals modifies sociability, but not control behaviours.
From: 5-HT release in nucleus accumbens rescues social deficits in mouse autism model
a, b, Quantification of chamber time (a: eYFP, F6,63 = 1.383, P = 0.2352, n = 8; ChR2, F6,72 = 4.891, P < 0.05, n = 9) and proximity time (b: eYFP, F3,42 = 0.9652, P = 0.4181, n = 8; ChR2, F3,48 = 7.565, P < 0.05, n = 9) in the three-chamber assay. c, d, Quantification of chamber time (c: eYFP, F6,81 = 1.626, P = 0.1506, n = 10; NpHR, F6,81 = 6.253, P < 0.05, n = 10) and proximity time (d: eYFP, F3,54 = 2.304, P = 0.0872, n = 10; NpHR, F3,54 = 7.821, P < 0.05, n = 10) in the three-chamber assay. e–g, Quantification of novel object interaction assay (e: F1,30 = 0.00206, P = 0.9641, n = 8–9), locomotion assay (f: F1,30 = 0.03023, P = 0.8631, n = 8–9), and centre time (g: F5,75 = 1.205, P = 0.3151, n = 8–9) in Sert-cre mice expressing DIO-eYFP or DIO-ChR2 in DR receiving DR-to-NAc terminal stimulation. h–j, Quantification of novel object interaction assay (h: F1,38 = 0.213, P = 0.6471, n = 10–11), locomotion assay (i: F1,34 = 1.077, P = 0.3066, n = 9–10), and centre time (j: F5,90 = 0.1646, P = 0.9749, n = 9–10) in Sert-cre mice expressing DIO-eYFP or DIO-NpHR in DR receiving DR-to-NAc terminal stimulation. Data are mean ± s.e.m. *P < 0.05, **P < 0.01; repeated measures, two-way ANOVA with Tukey’s multiple comparison post hoc test. Comparisons with no asterisk had P > 0.05 and were considered not significant.
