Extended Data Fig. 4: Activity analysis of disease-related and signalling-related mutations of ΔCRD-FZD4, and the conformational change of family-conserved amino acids of ΔCRD-FZD4.
From: Crystal structure of the Frizzled 4 receptor in a ligand-free state
a, TOPflash analysis, cellular localization and cell surface expression analysis of disease-related and signalling-related mutations. Each data point represents mean ± s.e.m., repeated in triplicate. The experiment was repeated using two independent methods with similar results. All the mutations affected FZD4 downstream signalling in some way—some decreased the WNT3A–Norrin signal substantially, some showed gain-of-function activity—which suggests complex mechanisms underlying these mutation-caused diseases. b, Conformational rearrangement on family-conserved residues W4947.55f and Y2502.39f was observed when comparing FZD4 (cyan) with SMO (gold). It is noteworthy that Y2502.39f in FZD4 points outwards from the 7TM bundle.
