Extended Data Fig. 1: Generation of HSP101–3×Flag P. falciparum and analysis of purified PTEX.

a, Schematic showing strategy for endogenous tagging of P. falciparum hsp101 with 3×Flag using CRISPR–Cas9 editing. Diagnostic PCR primers and expected amplicon following successful integration are shown. sgRNA, single-guide RNA; UTR, untranslated region; CAM, calmodulin promoter; PfU6, P. falciparum U6 promoter; hDHFR, human dihydrofolate reductase. b, Diagnostic PCR with genomic DNA template from NF54^attB parent or two independent populations of HSP101–3×Flag P. falciparum. The experiment was performed once. c, Western blot of NF54^attB and HSP101–3×Flag P. falciparum probed with mouse Flag M2 antibody (Sigma) and goat anti-mouse IRDye 680 secondary antibody (Li-Cor). Arrowhead indicates full-length HSP101–3×Flag (predicted molecular weight 102.9 kDa after signal peptide cleavage). Data represent two independent experiments. d, Giemsa staining of parasite-infected human erythrocytes from which PTEX was purified. Scale bar, 5 μm. For source data, see Supplementary Fig. 3. e, Silver-stained SDS–PAGE of the Flag-purified PTEX sample. Identities of the bands labelled EXP2, PTEX150 and HSP101 were confirmed by tryptic digest liquid chromatography–mass spectrometry (LC–MS). f, Tryptic digest LC–MS analysis of the Flag-purified PTEX sample. The PTEX core components are among the five most abundant species detected in the purified sample. For gel and blot source data, see Supplementary Fig. 1.