Extended Data Fig. 6: Confirmation of biphasic property of RANKL reverse signalling.
From: Coupling of bone resorption and formation by RANKL reverse signalling
a, Alizarin red staining of ST2 cells stimulated with mOC-SEVs, αR-tri, αB-tri, or vehicle during the early or late stage of differentiation. b, Alizarin red-stained ST2 cells infected with DN-Runx2 adenovirus before stimulation with αR-tri or αB-tri during the early or late stage of differentiation. c, d, Time courses of expression of early osteoblastic markers (Col1a1 mRNA and P1NP value reflecting the expression of type I collagen protein) and late osteoblastic markers (osteocalcin mRNA and Gla-osteocalcin protein) in mouse primary osteoblasts stimulated during the early stage (c) or late stage (d) of differentiation with mOC-SEVs, αR-tri, αB-tri, or vehicle (n = 3). e, f, Von Kossa-stained (e) or alizarin red-stained (f) mouse primary osteoblasts stimulated with mOC-SEVs, αR-tri, αB-tri, or vehicle during the early stage or late stage of differentiation. In c and d, data are mean, with individual points representing biologically independent samples.
