Extended Data Fig. 7: The effect of neutralizing mOC-SEVs.
From: Coupling of bone resorption and formation by RANKL reverse signalling
a, Influence of suppressing mRNA expression of Rab family members in osteoclasts on the secretion of vesicular RANK from mOCs (n = 3). b, Influence of suppressing mRNA expression of Rab family members in osteoclasts on formation of multinuclear cells (MNCs) and their mineral-resorbing activity (n = 3). c, Influence of GW4869 on the secretion of vesicular RANK from mOCs (n = 3). d, Influence of GW4869 on formation of MNCs and their mineral-resorbing activity (n = 3). e, Binding affinity of αI-tri1 or αI-tri2 to the IGSF8 ectodomain (n = 3 biologically independent samples). f, Influence of αI-tri1/2 on formation of MNCs and their mineral-resorbing activity (n = 3). g, Influence of pre-incubating mOC-SEVs with αI-tri1/2, αB-tri, GST–sRANKL or vehicle on the stimulatory activity of mOC-SEVs on ST2 cells; activation of the PI3K–Akt–mTORC1 pathway in ST2 cells is shown. In a–d, f, data are mean, with individual points representing biologically independent samples. Statistical analyses, one-way ANOVA followed by Student–Newman–Keuls test; *P < 0.05, **P < 0.01.
