Extended Data Fig. 9: Phenotypes of RANKLP29A mutant mice.
From: Coupling of bone resorption and formation by RANKL reverse signalling
a, Bone morphometric parameters in 17-week-old male RANKLP29A mice (n = 8) and wild-type littermates (n = 4) under physiological conditions. b, Bone morphometric parameters in OVX or sham operated 16-week-old female RANKLP29A mice (n = 7 (sham), 7 (OVX)) and wild-type littermates (n = 5 (sham), 5 (OVX)). c, Bone morphometric parameters in 8-week-old male RANKLP29A mice and wild-type littermates treated with daily subcutaneous injection of 20 or 100 μg kg−1 day−1 parathyroid hormone (PTH) over the course of 2 weeks (n = 3 in each group). d, Expression levels over time of RANKL and OPG mRNA in primary osteocytes derived from RANKLP29A mice and wild-type littermates stimulated with αR-tri or αB-tri (n = 3). e, TRAP activity in bone marrow macrophages (BMMs) and primary osteocyte co-culture derived from RANKLP29A mice and wild-type littermates (n = 3). f, Time-courses of the expression levels of sclerostin mRNA in primary osteocytes derived from RANKLP29A mutant mice and wild-type littermates stimulated with αR-tri or αB-tri (n = 3). In a–f, data are mean, with individual points representing biologically independent samples or mice. Unpaired two-sided Student’s t-test (a) or one-way ANOVA followed by Student–Newman–Keuls test (b–f.); *P < 0.05, **P < 0.01.
