Extended Data Fig. 2: Overlay of LepB–G0775 and LepB–arylomycin A2 (PDB ID: 1T7D).
From: Optimized arylomycins are a new class of Gram-negative antibiotics
Comparison of G0775 (coloured yellow) and arylomycin (coloured pink). The catalytic lysine 146 is covalently bound to the nitrile warhead, while the serine 91 nucleophile remains unbound. For simplicity, the LepB protein has been removed from each co-structure. The comparison indicates that the macrocyclic core of G0775 maintains high similarity to the parent arylomycin macrocycle, and makes very similar interactions with the protein.
