Extended Data Fig. 3: Flow cytometry of PBMCs sorted by alternative gating for CD32^hi, CD32^int and CD32^lo CD4 T cell populations, as well as T cell populations bearing markers of B cells (T–B) or other non-CD4-T-cells (T–other).

Cells in an inclusive light scatter gate consistent with either small lymphocytes or larger cells (first column) were enriched for single cells (second column). Within these gates, viable CD3⁺ cells (third column) that were CD19⁻ and CD20⁻ (lower gate, fourth column), CD16⁻ and CD14⁻ (fifth column), CD123⁻ (sixth column), CD4⁺ (seventh column), and CD32^hi, CD32^int or CD32^lo were then collected. Cells that were CD3⁺ and bearing markers of B cells (T–B; upper gate, fourth column) or other non-CD4-T-cells (T–other; combined ungated events from fifth and sixth columns) were also collected in separate tubes.