Extended Data Fig. 5: Flow cytometry, HIV DNA levels, and single-copy HIV DNA sequence analysis from CD32hi, CD32int and CD32lo CD4 T cell populations, and from T cells also bearing non-CD4-T-cell markers.
From: Conflicting evidence for HIV enrichment in CD32+ CD4 T cells
a, PBMCs from four additional study participants were collected from whole blood by venipuncture with immediate processing (without cryopreservation). The T–other population was collected as a combination of the ungated events from CD19/CD20, CD16/CD14 and γδ T cell receptor/CD123 exclusion plots (fourth, fifth and sixth columns). b, Left, copies of HIV DNA per million cells sorted from four additional study participants as in a. Right, percentages of all HIV DNA copies detected in blood cells deriving from CD32hi, CD32int, CD32lo and T–other subsets, calculated by adjusting values in the left panel for the relative proportions of these subsets determined using FACS data. c, Sequences of individual HIV DNA copies were determined by Sanger sequencing of products obtained by fluorescence-assisted clonal amplification, which amplifies a region of the HIV env gene. Phylogenetic trees were constructed as described in the Supplementary Methods. All Bonferroni-corrected Slatkin–Maddison P values for genetic compartmentalization between any two subsets were greater than 0.05 in all four participants.
