Extended Data Fig. 5: Flow cytometry quantification of nodes downstream of CD6 signalling endodomains and high-throughput analysis of super-resolution imaging using deconvolution microscopy.
From: A homing system targets therapeutic T cells to brain cancer
a–c, Quantification of the flow cytometric data for LFA-1 open configuration (a), pZap70 (b) and talin (c) before (solid bars) and after (dotted bars) TWM of 1 × 105 T cells. ***P < 0.001. d, Characterization of cellular features of migrant T cells using collective quantification of actin MFI, focal adhesions at HS–ALCAM interface, area of spreading, and podosynapse formation by high-throughput microscopy in three donors. n = 200–800 cells. e, Box plot summary representing single-cell data distributions of all replicates between all three donors expressing HS versus NT controls. Centre lines, data median. Boxes, middle quartiles. Whiskers, upper and lower limits.
