Extended Data Fig. 7: Analysis of T cell infiltrates in vital organs and normal brain after infusion of HS T cells.
From: A homing system targets therapeutic T cells to brain cancer
a, CD3 immunohistochemistry (IHC) staining of normal vital tissues from animals receiving HS T cells or NT control cells. n = 3 mice per group. Scale bars, 40 μm. b, IHC showing HS T cell infiltrate in micro-dissected GBM xenograft. Scoring of CD3+ DAB signal was analysed using IHC-Profiler plugin in ImageJ. Respective image analysis output and the score assigned using IHC-Profiler are also shown for each image. Total percentage of CD3+ DAB signal was more 66% in all mouse brain with HS T cells (scores 3–4) and percentages in control mice were less than 20% (scores 0–1). Scale bars, 50 μm. n = 3 mice per group.
