Extended Data Fig. 8: Characterization of therapeutic T cells after transmigration through an in vitro BBB model.
From: A homing system targets therapeutic T cells to brain cancer
a, Flow cytometry assessing HER2-CAR and HS molecule expression in HS HER2-CAR T cells. b–d, 1 × 105 T cells were collected from the bottom chamber after transmigration on ALCAM-expressing endothelium and analysed for CD45RO and CCR7 to assess their centrality (b), expression of the exhaustion markers PD-1 (black), TIM-3 (red) and LAG3 (orange) (before transmigration is shown in grey) (c), and proliferative capacity before (red) and after (blue) transmigration, using efLuor 670 (d).
