Extended Data Fig. 1: Analysis of CAM expression in primary brain tumours.
From: A homing system targets therapeutic T cells to brain cancer
a, High-throughput IFC analysis of the endothelial adhesion molecules ICAM1, VCAM1, and ALCAM in 93 primary GBM, 25 primary medulloblastoma and 5 normal brain samples. MATLAB segmentation and masking analysis algorithm of the co-immunofluorescence (co-IFC) of ICAM1 or VCAM (acquired on 647 channel), CD31 (acquired on 594 channel) and ALCAM (acquired on 488 channel), and DAPI (acquired on blue/cyan channel). b, Isolation and characterization of pTECs. Flow cytometry sorting gating strategy of pTECs from freshly excised glioblastoma (GBM; n = 5) based on CD31 positivity. Isolated GBM endothelial cells also expressed the endothelial markers VE-cadherin, von Willebrand Factor (vWF) and ALCAM. Isotype shown in lighter grey and test shown in darker grey in individual histograms. n = 5 surgical samples each interrogated at least twice. At least 100,000 events were acquired per condition.
