Extended Data Fig. 2: ALCAM expression in a panel of human and mouse endothelial cells and their reactivity to inflammatory and cancerous conditioning.
From: A homing system targets therapeutic T cells to brain cancer
a, Western blot for ALCAM in a panel of human and mouse endothelial cell lines: pTECs, HBMECs, 1ry BMECs, 1ry PVECs, HUVECs, HMVEC-Ls, bEnd.3 (mouse brain tumour EC) and 2-H11 (mouse SV40-transformed axillary lymph node vascular endothelium). Left, basal ALCAM expression except in tumour endothelial cells (pTEC and 2-H11). Right, induction of ALCAMs in all endothelial cells after incubation with TNFα for 6 h. b, Expression of ALCAM at baseline and after 6 h of conditioning in GBM supernatant, TGFβ or IL6. Only tumour endothelial cells expressed ALCAM at baseline while normal endothelial cells did not. c, IFC for ALCAM in 5 × 104 pTECs and HBMECs in the in vitro BBB model at baseline and after culture in GBM supernatant. Scale bars, 50 μm. d, Differential expression of key adhesion molecules at baseline and under the influence of cancer and inflammation in pTECs and HBMECs. Flow cytometry dot plots detailing baseline expression of ALCAM, VCAM1 and ICAM1 on 1 × 104 pTECs and HBMECs and conditioned expression after culture in GBM supernatant, TGFβ or IL6. e–h, Expression of adhesion molecules at baseline and under the influence of cancer and inflammation in pTECs (n = 4) acquired from surgically resected samples (pTEC #1 is shown in Fig. 1g).
