Extended Data Fig. 7: Neuronal activity in cortical and subcortical areas upon light touch after SNI.
From: Touch and tactile neuropathic pain sensitivity are set by corticospinal projections
a–c, Drawings of c-Fos immunostaining in intact mice (a), mice with SNI only (b) and mice with SNI after light brush stimulation (c) from control mice and mice with pyramidotomy. mPFC, medial prefrontal cortex; ACC, anterior cingulate cortex; S1HL, hindlimb primary somatosensory cortex; S2, secondary somatosensory cortex; Pir, piriform cortex; PV, periventricular nucleus of the thalamus; VM, ventromedial nucleus of the hypothalamus; Amyg, amygdala. d, Quantification of c-Fos+ cells in multiple cortical areas in intact mice (with CSN, n = 3; with pyramidotomy, n = 3), mice with SNI only (with CSN, n = 3; with pyramidotomy, n = 3), and mice with SNI after light brush stimulation (with CSN, n = 4; with pyramidotomy, n = 3). **P < 0.01; n.s., no statistical significance. PFC SNI only with or without Py: P > 0.99; PFC SNI + brush with or without Py: P < 0.0001; ACC SNI only with or without Py: P > 0.99; ACC SNI + brush with or without Py: P = 0.0002; M1 SNI only with or without Py: P > 0.99; M1 SNI + brush with or without Py: P > 0.99; S1 SNI only with or without Py: P > 0.99; S1 SNI + brush with or without Py: P < 0.0001; insula SNI only with or without Py: P > 0.99; insula SNI + brush with or without Py: P < 0.0001; S2 SNI + brush with or without Py: P = 0.0075; Pir all conditions (ANOVA): P = 0.82. One-way ANOVA followed by Bonferroni correction. e, Representative images of multiple cortical areas stained with c-Fos (red) and GFP (green, HiRet-GFP injection) in mice with SNI after light brush stimulation. Arrowheads mark the co-localization of c-Fos+ and GFP+ CSNs. Scale bars, 20 μm. f, Quantification of c-Fos+ and GFP+ CSN co-localization in multiple cortical areas in animals with SNI after light brush stimulation without (n = 4) or with pyramidotomy (Py, n = 3). *P < 0.05; **P < 0.01, P = 0.03, 0.04, 0.0009, and 0.02 for mPFC, ACC, S1, and S2, respectively. Two-sided Student’s t-test. Data shown as mean ± s.e.m.
