Extended Data Fig. 8: Ablation of lumbar CCK+ interneurons reduces tactile sensitivity and dorsal horn neuronal activation after SNI, but not nociceptive response or gross locomotion.
From: Touch and tactile neuropathic pain sensitivity are set by corticospinal projections
a–d, Measurements of sensitivity to laser heat (a, P = 0.76), acetone (b, P = 0.86), von Frey filaments (c, P = 0.03 for 0.016 g) and brush (d, P = 0.002) stimuli in control mice (n = 8) or mice in which CCK–tdTomato interneurons had been ablated (n = 7). a, b, d, Two-sided Student’s t-test; c, two-way repeated measures ANOVA followed by Bonferroni correction. e, f, Performance on open field (e, P = 0.54) and ground walking (f, P = 0.68, 0.72, and 0.50 for hindlimb weight support, protraction, and retraction, respectively) in control mice (n = 8) or mice in which CCK–tdTomato interneurons had been ablated (n = 7). n.s., no statistical significance, two-sided Student’s t-test. g–i, Representative images of c-Fos (green) activity (g) and quantification of CCK+/c-Fos+ cells (h) and c-Fos+ neurons in different laminae of the dorsal horn of the spinal cord (L3–4) of CCK–tdTomato (red) mice after SNI and brush stimulation in mice that had undergone sham surgery (n = 4), pyramidotomy (n = 5) or lumbar CCK–tdTomato ablation (n = 3) (i). Scale bars, 500 μm (g, right, middle, and left columns) and 50 μm (g, zoom in panels). **P < 0.01, two-sided Student’s t-test. h, P < 0.0001; i, P = 0.0045 and 0.0028 for laminae I–II and laminae III–V, respectively. Data shown as mean ± s.e.m.
