Extended Data Fig. 10: Reinforcement of tactile allodynia in mice with SNI by optogenetic stimulation of somatosensory CSNs.
From: Touch and tactile neuropathic pain sensitivity are set by corticospinal projections
a, Drawing of experimental paradigm. b, c, Measurement of punctate (b, von Frey filaments) and dynamic (c, brush) mechanical allodynia upon opto-stimulation in control mice (n = 6) and mice expressing ChR2-YFP in CSNs (n = 6) after SNI. n.s., no statistical significance; *P < 0.05. b, c, P = 0.18, 0.08 and 0.41, 0.02 for PLAP and Cre, without or with laser, respectively; two-sided Student’s t-test. d, Representative images and quantification of pERK (red) and NK1R (green) immunostaining in the superficial dorsal horn (laminae I–II) of the spinal cord (L3–4) in control mice (n = 3) or mice expressing ChR2-YFP in hindlimb CSNs (n = 3) with SNI and brush stimulation coupled with opto-stimulation. Scale bar, 100 μm. *P < 0.05; P = 0.02 and 0.01 for pERK and pERK/NK1R ratio, respectively; two-sided Student’s t-test. Six sections crossing the lumbar spinal cord (L3–4) were quantified for individual animals. Data shown as mean ± s.e.m.
