Extended Data Fig. 7: Mechanically induced release of angiocrine signals.
From: Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival
a, a′, b, b′, c, c′, d, d′, e, e′, f, f′, h, h′, i, i′, Unstretched versus mechanically stretched human hepatic ECs, stained for F-actin (a′, b′, c′, d′, e′, f′, h′, i′), activated β1 integrin (a, a′,b, b′) and shown as PLA dots: VEGFR3 tyrosine phosphorylation (c, c′, d, d′), co-localization of β1 integrin and VEGFR3 (e, e′, f, f′) and VEGFR2 tyrosine phosphorylation (h, h′,i, i′). g, j, Quantification of the interaction between VEGFR3 and β1 integrin (n = 3 unstretched chambers versus n = 4 stretched chambers, *P = 0.0091 (0.99; 4.16)), and VEGFR2 tyrosine phosphorylation (n = 4 stretch chambers each, P = 0.4000 (−0.37; 0.19)). k, IL-6 protein concentration (n = 4 stretch chambers each, *P = 0.0191 (14.51; 109.70)). l, TNF protein concentration (n = 4 unstretched chambers versus n = 5 stretched chambers, *P = 0.0493 (0.43; 192.40)). m, MMP9 activity (n = 4 stretch chambers each, *P = 0.0488 (0.44; 111.20)), in the supernatant of unstretched versus stretched hepatic ECs. Scale bars, 10 μm. Data are mean ± s.e.m. Two-tailed unpaired Student’s t-tests, 95% confidence interval (lower confidence limit; upper confidence limit).
