Extended Data Fig. 3: Endothelial β1 integrin is required for hepatic cell proliferation and survival in the liver periphery.
From: Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival
a, a′, b, b′, d, d′, e, e′, Transversal sections through E11.5 livers from EC-specific heterozygous (control) versus homozygous (β1 integrin-null) knockouts of β1 integrin, including magnifications (a′, b′, d′, e′) after 3 h EdU incorporation in WEC. c, Quantification of EdU-positive peripheral areas: n = 4 control versus n = 3 β1 integrin-null livers (*P = 0.0207 (19.06; 93.54)). f, Ki-67-positive cells in embryonic livers: n = 6 Cre-control versus n = 6 β1 integrin-null livers (P = 0.0666 (3.71; −86.50)). g, g′, g′′, h, h′, h′′, i, i′, i′′, Transversal sections through control (g, g′, g′′) and β1 integrin-null (h, h′, h′′) E13.5 livers with vascular-painted (RCA), perfused hepatic vessels, including magnified images (i, i′, i′′). Scale bars, 100 μm (a, b, d, e), 50 μm (a′, b′, d′, e′, g, g′, g′′, h, h′, h′′) and 25 μm (i, i′, i′′). Data are mean ± s.e.m. Two-tailed unpaired Student’s t-tests, 95% confidence interval (lower confidence limit; upper confidence limit).
