Extended Data Fig. 4: Cdh5-creERT2-mediated depletion of β1 integrin reduces liver growth, survival, VEGFR3 activation and HGF production.
From: Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival
a, a′, b, b′, E13.5 mouse embryos (a′, b′), with their abdomen magnified (a, b). c, Calculated volumes of livers taken from mouse embryos with Cdh5-creERT2-mediated depletion of β1 integrin (labelled ‘Cdh5-β1−/−’) and control littermates (n = 3 embryos each, *P = 0.0446 (0.15; 5.03)). d, d′, e, e′, Transversal sections through E13.5 livers with magnifications (d′, e′). f, Quantification of cleaved caspase-3-positive areas (n = 3 embryos each, *P = 0.0462 (12.62; 634.90)). g, VEGFR3 tyrosine phosphorylation normalized to total VEGFR3 (n = 4 control embryos versus n = 4 embryos with Cdh5-creERT2-mediated depletion of β1 integrin, *P = 0.0445 (1.81; 91.90)). h, HGF protein concentrations normalized to total protein (n = 16 control embryos versus n = 9 embryos with Cdh5-creERT2-mediated depletion of β1 integrin, *P = 0.0030 (8.81; 36.46)). Scale bars, 500 μm (a, b), 1 mm (a′, b′), 200 μm (d, e) and 50 μm (d′, e′). Data are mean ± s.e.m. Two-tailed unpaired Student’s t-tests, 95% confidence interval (lower confidence limit; upper confidence limit).
