Extended Data Fig. 1: Amino acid sequences of the CGRP peptide, CLR and RAMP1 constructs used for determination of structure.
From: Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
The sequences are annotated to denote the location of the haemagglutinin (HA) signal sequence (red highlight), 3C cleavage sites (grey highlight), Flag (dark olive-green highlight) and His tags (purple highlight). The substituted sequences of the native proteins are listed above the construct sequences and highlighted in blue. Transmembrane helical domains in CLR and RAMP1 are boxed and highlighted in green. Segments of the proteins that were not resolved in the cryo-EM map are highlighted in yellow. Amino acids for which backbone density was present but there was limited side-chain density were stubbed in the model; these are shown in bold red in the sequences.
