Extended Data Fig. 2: CGRP receptor pharmacology and purification of the CGRP–CLR–RAMP1–Gs complex.
From: Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
a, Pharmacology of untagged CLR–RAMP1 (wild-type (WT) CLR–RAMP1) and the purification construct (HA–Flag–CLR and Flag–RAMP1), in CGRP-mediated cAMP accumulation assays performed in transiently transfected COS-7 cells. n = 5 independent experiments with triplicate repeats; data are mean + s.e.m. b, Expression and purification strategy. c, Final size-exclusion chromatography elution profile of the complex. d, SDS–PAGE and Coomassie blue staining of the size-exclusion chromatography peak, demonstrating the presence of each of the components of the complex.
