Extended Data Fig. 5: Alignment of modelled active complex and X-ray structure.
From: Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor
Backbone of the ECD of CLR (blue ribbon) and RAMP1 (orange ribbon) from the modelled, active complex, and the structure of the isolated CLR–RAMP1 ECD complex solved by X-ray crystallography12 (light grey ribbon). The structures were aligned on the RAMP1 ECD. The CLR loops (loops 1–5) are annotated. The CLR loop 1 and loop 5 sequences that were not resolved in the cryo-EM map are indicated by dotted black arrows. Differences in the backbone position of CLR loops 4 and 5 are indicated in blue (active complex) and grey (isolated ECD complex) dotted arrows. The location of the CGRP peptide is shown in dark red.
