Extended Data Fig. 6: Widespread role of METTL3 in oncogene translation.

a, Immunoprecipitation of endogenous METTL3 and western blotting using the indicated antibodies. The blot is representative of two independently performed experiments with similar results. b, Density plot reflects the distribution of changes in percentage spliced in ∆PSI values and corresponding P values for alternative splicing events detected by rMATs v.3.2.5 (rMATs is developed on the basis of a hierarchical framework and likelihood-ratio test was used to detect differential splicing). Splicing events at a FDR < 5% and ΔPSI > 0.1 are considered as significant. Black dots indicate total mRNAs. Red dots (4,276 mRNAs) indicate mRNAs in METTL3-depleted cells that are translated more than twofold less. c, Western blot using indicated antibodies in control-, METTL3- or YTHDF1-knockdown cells. Blots are representative of two independently performed experiments with similar results. d, RT–qPCR analysis of endogenous BRD4 mRNAs. Data are mean ± s.e.m. from three biologically independent samples. e, Annexin V/PI staining of METTL3 knockdown and control A549 cells upon JQ1 treatment (analysed by FACS). Data are from three independent experiments.