Extended Data Fig. 5: Methodological comparison of detected genes and library saturation.
From: Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris
a, The number of genes detected (threshold of >0 reads or UMIs per cell) by FACS (red; n = 21,105 individual cells), microfluidic-droplet (green; n = 55,032 individual cells) and microwell-seq (blue; n = 25,891 individual cells) methods20. b, Library saturation fraction for all microfluidic-droplet libraries. Dotted horizontal line demarcates the median saturation (around 0.9). c, Library saturation for all FACS libraries. Saturation was calculated using the number of detected genes while downsampling the number of reads per library. Summary statistics are contained in Supplementary Table 6.
