Extended Data Fig. 6: IRE1α–XBP1 activation and ER stress responses in ovarian cancer-associated T cells isolated from mouse models of disease.

a, b, Expression of marker genes (Xbp1, Xbp1s, Hspa5 and Ddit3) for ER stress was determined by qPCR. Circles, CD4⁺ T cells; squares, CD8⁺ T cells. a, CD45⁺TCRβ⁺CD4⁺ and CD45⁺TCRβ⁺CD8⁺ cells were sorted from tumours (n = 8), spleens (n = 4) and lymph nodes (n = 4) of mice bearing advanced ovarian tumours driven by p53 and KRAS. b, CD45⁺TCRβ⁺CD4⁺ and CD45⁺TCRβ⁺CD8 cells were isolated from malignant ascites (n = 6), spleens (n = 8) and lymph nodes (n = 8) of mice bearing aggressive ID8-Defb29/Vegfa ovarian cancer (OvCa), and from spleens (n = 6) of naive mice as control. c, d, Expression of target genes (Bloc1s1 and Gm2a) of canonical regulated IRE1α-dependent decay (RIDD)—in CD4⁺ and CD8⁺ T cells isolated from different tissues of mice that bore ovarian cancer—was analysed by qPCR. c, T cells were sorted from tumours (CD4⁺ T cells, n = 5; CD8⁺ T cells, n = 4), spleens (n = 2) and lymph nodes (n = 2) of mice bearing advanced ovarian tumours driven by p53 and KRAS. d, T cells were isolated from malignant ascites (CD4⁺ T cells, n = 8; CD8⁺ T cells, n = 6), spleens (n = 13) and lymph nodes (n = 8) of mice that bore aggressive ID8-Defb29/Vegfa ovarian cancer. Data were normalized to Actb. Data are shown as mean ± s.e.m. (a–d). n values represent biologically independent samples (a–d). One-way ANOVA with Tukey’s multiple comparisons test (a, b); *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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