Extended Data Fig. 7: Molecular model and controls showing that p300 acetyltransferase activity is not stimulated by eRNA.

a, p300 is maintained in the inactive state by deacetylases such as SIRT2. IRF3 is autoinhibited by a C-terminal segment in the IAD domain. b, TBK1 phosphorylation activates and dimerizes IRF3. The activated IRF3 dimer engages the IBID domain of p300. c, Recruitment of two molecules of p300 results in trans-autoacetylation in the AIL loop and HAT activation. d, Activated p300 can acetylate chromatin and engage acetylated substrates via the bromodomain. e, A constant amount of p300s (2 μM) was incubated in [¹⁴C]acetyl-CoA alone or in the presence of 2 μM Klf6 eRNA for the indicated time points. Samples were analysed by SDS–PAGE followed by Coomassie staining (top) and autoradiography (bottom). f, As in e but in the presence of 0.5 mM EDTA. The experiment was carried out at least twice with consistency. One representative example is shown. g, Quality control of Klf6 RNA. 3 μg Klf6 was deposited on a 1% agarose gel or a 14% 6 M urea PAGE gel and detected by SYBR Safe stain. M, 100-bp DNA ladder.