Extended Data Table 2 Thermodynamic analysis of the interaction between p300 BRP and histone peptides by ITC

From: Transcription factor dimerization activates the p300 acetyltransferase

  1. Mean and s.d. were determined from experiments performed in triplicate. Horizontal lines separate experiments involving different histone peptides or different protein constructs.
  2. Histone peptide sequences: H3 (1–20) ARTKQTQRKSTGGKAPRKQL, H3 (11–30) TGGKAPRKQLATKASRKSAP, H4 (4–24) GKGGKGLGKGGAKRHRKVLRD.
  3. AIL (Autoinhibitory loop peptide) SKNAKKKNNKKTSKNKSS (1545–1562). No binding was detected to non-acetylated peptides, or with a construct containing a mutation that abolishes acetyllysine binding (N1132A).
  4. *Binding stoichiometry.
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