Extended Data Fig. 1: Differentially expressed genes in single-cell and bulk RNA-seq.
From: Distinct descending motor cortex pathways and their roles in movement
a, b, Heat map of expression (from scRNA-seq) of differentially expressed genes identified from scRNA-seq (a) and bulk RNA-seq (b). Columns represent individual cells, grouped by transcriptomic cluster (indicated below each colour map; Slco2a1, n = 203; Npsr1, n = 43; Hpgd, n = 122) and retrograde labelling site (indicated above). Grey indicates cells isolated from transgenic lines or other targets. Rows represent genes differentially expressed between the Slco2a1 and Hpgd–Npsr1 clusters. Colour bar shows transcript expression in counts per million mapped reads (CPM + 1) on a log-scale. c, d, Heat map of expression (based on bulk RNA-seq) of differentially expressed genes identified from scRNA-seq (c) and bulk RNA-seq (d) datasets. Rows represent genes, coloured by differential bulk RNA-seq expression between thalamus-labelled and medulla-labelled PT neurons. Columns in the heat map represent individual replicates (six each for PTupper and PTlower). Colours show log-transformed transcript intensity (CPM + 1) in z-scored units. Blue shows replicates with low expression (z-score = −2); red shows replicates with high expression (z-score = +2).
